Coding

Part:BBa_J100109:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-04-18)

adhE optimized for E. coli, PstI removed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2691
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2692
    Illegal PstI site found at 2706
    Illegal NotI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2692
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2692
    Illegal PstI site found at 2706
    Illegal AgeI site found at 591
    Illegal AgeI site found at 1815
    Illegal AgeI site found at 1860
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7


Design Notes

Had to remove 4 internal PstI sites. Codons were optimized for E. coli.


Source

gi|49175990:1294669-1297344 Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome Reverse strand.

References